New Product Introduction | Aizin Bio-SpheroNex Cell Spheroid Culture 6-well Plate Set

Traditional 2D cell culture is difficult to reduce the real interaction of cells in vivo, there are research limitations

3D cell culture is closer to the tissue state in vivo by constructing cell spheres.

It has become an important tool in drug screening, disease research and other fields, and has also spawned the need for efficient 3D cell ball construction tools.

The core applications of 3D cell culture technology cover drug screening and toxicity testing, disease modeling and efficacy evaluation, regenerative medicine and tissue engineering.

The direction of cell differentiation research and process development is an important boost to current scientific research, targeting the needs of high-throughput, high-stability 3D cell culture.

Aizin biological research and development launched SpheroNex cell spheroid culture 6-well plate set, can achieve a variety of cell types of efficient, stable ball,

professional solutions for 3D cell culture.

PART. 01 Product Introduction

aizin SpheroNex^™ Cell sphere culture 6-well plate set is a professional product designed for# high-throughput 3D cell culture needs

it is suitable for efficient and stable ball formation of various cell types.

Storage and stability ( Storage and Stability)

PART. 02 Core advantages

1. High yield and high efficiency, suitable for high throughput demand

using a unique micro-hole array design, each hole contains 1500 micro-holes with a pore size of 800μm

single hole can be prepared 1500 cell microspheres, a single piece of 6 well plate can be prepared up to 9000 cell microspheres.

2. Uniform and controllable to ensure data reliability

through precise micropore size and anti-adhesion coating technology, cells are evenly aggregated in a defined space, and the uniformity of cell ball size and roundness is excellent.

The experimental results showed that stable and uniform MSC cell microspheres could be formed in 24 hours.

3. Convenient operation, reduce the risk of pollution

without centrifugation, the cells can be pelleted by static culture after adjusting the seeding density, simplifying the process and reducing the risk of cell exposure and contamination.

If you need to accelerate the ball, you can choose 100xg, 3 minutes low-speed centrifugation, flexible adaptation of different programs.

4. Wide adaptability, covering multiple types of cells

compatible with strongly adherent cells (such as HELA cells), weakly adherent cells (such as 293T cells), adherent stem cells (such as MSC mesenchymal stem cells), and suspension cells (such as JURKAT cells)

the inoculation density range is 1.0 × 10 ² ~ 2.0 × 10 ³/micropore (I. e. 1.5 × 10 ³ ~ 3.0 × 10 ³/pore), which meets the needs of individual experiments.

PART. 03 Operation process

01 Package Preparation

add 1.5mL of anti-adhesion coating solution to each well, stand in the incubator for 10 minutes, discard the solution, rinse with sterile water or PBS, and dry for later use.

02 Cell inoculation and culture

add 4~5 mL of cell suspension with suitable density to each well, incubate in incubator for 24~48 hours, and then obtain uniform and compact cell spheres;

03 Operation Precautions

the whole operation is gentle, to avoid cell ball string hole, damage or loss;

Slowly suck 2~3mL of the old culture medium along the wall of the hole when changing the liquid, and then gently add the same volume of preheating new culture medium;

when collecting the cell ball, transfer it to a pointed centrifuge tube with a 5mL pipette, remove the supernatant after static sedimentation, and carry out subsequent experiments after confirming that the ball is tight.

PART. 04 Frequently Asked Questions

q1: Conditions of Use of Coating Solution after Opening?

A1: It is recommended to refrigerate at 2~8 ℃ after opening, avoiding high temperature or direct sunlight.

Q2: What is the recommended density range for cell inoculation?

A2: cell type, target cell ball size adjustment,

it is recommended to be 1.0 × 10 ² ~ 2.0 × 10 ³/micropore (I. e. 1.5 × 10 ³ ~ 3.0 × 10 ³/pore).

Q3: Do you need centrifugation during culture?

A3: No centrifugation is required, and the ball can be formed by standing still. After the culture plate can be closed,

centrifuge at 100x g for 3 minutes at low speed, and then return to the incubator for incubation.

Q4: How to deal with uneven cell microsphere size?

A4: Shake the cells as soon as possible after inoculation to ensure that the incubator is placed horizontally to avoid uneven cell aggregation caused by gravity.

Q5: How to exclude bubbles generated during the coating process?

A5: Tap the culture plate or gently blow with a pipette gun; if not removed, 1000xg, 1 minute high-speed centrifugation can be used.

PART. 05 aizin guards the experiment without worry

aizin Biology focuses on the research and development and production of experimental tools for scientific research, with the mission of "serving the cause of human health and contributing to life science research"

with professional technical team and strict quality control system, we provide high-quality products and technical support for global researchers.

Aizin SpheroNex^™ Cell sphere culture 6-well plate set, with technological innovation to empower scientific research innovation, help researchers in the field of# 3D cell culture to achieve more breakthrough results!

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