Product Recommendation | Worried about low efficiency and batch-to-batch variability when generating cell spheroids? This domestically produced 3D culture plate is simply game-changing!

Are researchers who do cell sphere culture always troubled by the problems of uneven microspheres,complicated operation and sample loss?

Don't worry! Aizu biological independent research and development SpheroNex™Cell sphere culture 6-well plate set, from design to reagent all-round optimization, easily crack the pain point of conventional culture plate experiment, and create an efficient and stable solution for 3D cell culture!

This culture plate uses an innovative microporous matrix with an exclusive anti-adhesion coating solution to ensure the uniformity of microspheres from the source,

at the same time, it simplifies operation, reduces loss, and adapts to various cell and experimental needs. It is a practical weapon for 3D cell experiments such as drug screening, tumor model construction, and stem cell culture.

Part1 four core advantages, help to achieve efficient and stable microsphere preparation

1. Microporous matrix precise shape, uniform and stable quality of microspheres

each hole contains 1500 exclusive microporous matrix with a pore size of 800 microns, which delimits the fixed space for cell aggregation and realizes "single microporous into single microsphere",

to improve the problem of irregular aggregation of cells in conventional culture plates, the cultured microspheres are consistent in size and uniform in shape, which improves the repeatability of experiments and makes the data more convincing.

Figure 1 under the same inoculation density, medium formula and culture environment, the formation of MSC cells was dynamically observed for 24h,

(A) Aizin SpheroNex ^TM cell balls cultured on culture plate sets (B) cell balls cultured on foreign h brand culture plates (c) cell balls cultured on foreign s brand culture plates.

The results showed that Aizin 6-well plate and foreign similar products can induce cells to form uniform and stable cell microspheres, and there is no significant difference in the quality of the ball.

Figure 2 aizin and other brands of products cultured MSC 24h collected cell ball. (a) Aizu;(B) S brand products; (c) H brand.

The results showed that Aizin cells into anti-adhesion matrix 6-well plate can obtain a large number of cell balls at one time, and the quality uniformity is good, and there is no significant difference compared with S brand, H brand and other similar products.

2. Nano-coated technology, efficient ball adaptation

according to the growth characteristics of cells in vitro culture, they can be divided into suspension cells and adherent cells, in which the adherent strength of different adherent cells is also different, for example, the strong adherent Hela cells can still grow on the surface of non-TC-treated cell culture.

However, the anti-adhesion coating liquid matched with this product adopts nanotechnology to form an ultra-thin hydrophilic coating on the surface of the microplate, which greatly reduces the adhesion between cells and the plate wall,

help cells quickly and orderly aggregation, 24~48h can form a stable cell sphere, widely adapted to strong adhesion, weak adhesion, suspension and other all types of cells.

Figure 3 Cell sphere morphology formed by culturing cells with different adherent strengths for 24h using this product.

(A) strongly adherent-Hela ;(B) weakly adherent -293T ;(C) adherent-MSC;(D) suspended-Jurkat. The results showed that no adherent phenomenon was found in all kinds of cells, and the cells spontaneously formed cell aggregates.

3. The inoculation density is wide and adjustable, and the microsphere size is precisely controllable.

Cell seeding density is a key parameter to regulate the morphological uniformity, size and functional activity of 3D cell spheres, which directly affects the reliability of subsequent drug screening and toxicological evaluation experiments.

It has been verified that each inoculation density of this product can be stable into a ball, with a wide range of inoculation density,

and the larger the seeding density, the larger the cell sphere (the recommended cell seeding density range is 1.0 × 10 ² ~ 2.0 × 10 Å/microwell, I .e. 1.5 × 10 5~3.0 × 107/well, and the cell suspension volume is 4~5 mL/well).

The size of the cell microsphere product can therefore be controlled by adjusting the number of cells seeded.

Figure 4 this product is inoculated with different densities (500, 1000, 2000, 4000 cells/microwells) of MSC and Jurkat-cloneE6-1 the effect of cell formation.

4. No centrifugal simplify operation, reduce the risk of pollution

the product adopts the static culture into ball design, only need to adjust the cell inoculation density, do not need to centrifuge into ball, the matching package process is also standardized and simplified, novice can quickly get started.

The whole process reduces the tedious operation steps, avoids the cells from repeatedly entering and leaving the sterile barrier, reduces the risk of pollution, and saves the experiment preparation time.

Figure 5 the ball formation of the static incubation group of directly inoculated cells and the incubation group after slow centrifugation (100 × g 3 min).

(A) Static incubation group;(B) Centrifugal process group. The results showed that the cells in the centrifugation process group could initially form microspheres after centrifugation;

after 24 h incubation, the static incubation group could also form stable cell microspheres, and the morphology was not significantly different from the centrifugal process group.

Part2 minimalist standardized process, novice can easily get started

aizin Biological SpheroNex™The operation process of the 6-well plate set for cell sphere culture has been optimized for many times, and the preparation of microspheres can be completed by basic laboratory operation without complex instruments:

1. Standardize the orifice plate with anti-adhesion coating solution, and fully dry it for later use;

2. Add the cell suspension of suitable density to the well plate;

3. Place it in an incubator for static culture for 24-48 hours to obtain uniform and stable cell microspheres;

4. After collecting the microsphere suspension, the downstream experiment can be carried out directly. For detailed operation specifications, please refer to the supporting experimental guidelines. For specific operation steps, please refer to [Part 3 Operation Steps] below].

Part3 multi-scene adaptation scheme to meet diverse 3D training needs

aizin Biological SpheroNex^™ Cell sphere culture 6-well plate set, with innovative microporous matrix design and nano anti-adhesion coating technology,

it provides high-throughput, high-uniformity, and high-adaptability solutions for 3D cell culture. It has good performance in ball quality, ease of operation and sample protection, and is suitable for a variety of 3D cell culture research scenarios.

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