What is the difference between automated tip and pipetting tip? Selection of full strategy

1. what is an automated tip?

Automated tips (Automation Tips) is applied automated Pipetting Workstation the core consumables are used to achieve precise extraction and distribution of liquids.

Unlike traditional manual pipetting tips, automated tips are designed manipulator Operation Design widely used in:

· Nucleic acid extraction (DNA / RNA)

· Construction of PCR / qPCR system

· High-throughput screening

· Biopharmaceutical R & D

in essence, it is one of the most critical execution components in an automated experimental system

the working principle of 2. automatic suction head

the automated tip works with the pipetting workstation:

(1) Absorption (negative pressure formation)

(2) Liquid separation (precise release)

(3) Multi-channel synchronous operation

(4) High-throughput batch processing

the entire process is controlled by the program to achieve high precision liquid transfer

3. automatic suction head vs ordinary suction head (core difference)

conclusion: automated experiments must use automated tip, otherwise the accuracy can not be guaranteed

4. the core types of automated tips

(1) classification by capacity

common specifications:

• 10 μL
• 20 μL
• 50 μL
• 200μL
• 1000μL

different volumes adapt to different experimental requirements

(2) Classification by structure

• with filter tip (Filter Tips)

anti-aerosol pollution

suitable for PCR/nucleic acid experiments

• suction head without filter element

lower cost

suitable for routine experiments

(3) Classification by function

• Low suction tip (Low Retention)
• conductive suction head (suitable for liquid level detection)
• DNA/RNase-free tips (for molecular experiments)

key performance indicators of 5. automated tips

when choosing an automated tip, you must pay attention to the following core indicators

✔1. Material (core)

the mainstream is medical grade PP (polypropylene)

advantages:

· Strong chemical stability

· Low dissolution

· Suitable for high-throughput experiments

✔2. Accuracy and consistency

automated experiments require extremely high pipetting accuracy:

• Batch consistency

· Precise control of trace liquid

otherwise, the experimental results will be biased.

✔3. Tightness and adaptability

high matching with equipment (e. g. Hamilton / Tecan, etc.)

avoid liquid leakage and suction

automated tips must be "fully compatible" with the device"

✔4. Cleanliness (very critical)

high-quality automated tips need to meet:

· DNA-free enzyme

· No RNA enzyme

· No heat source

· No protease

usually in the clean workshop production

the core role of 6. automated tip in the experiment

1. Improve experimental efficiency

supports 96 channels or even 384 channel operation for high throughput processing

2, improve the accuracy of the experiment

reduce human error and ensure consistent pipetting every time

3. Reduce the risk of pollution

filter element design can effectively prevent aerosol pollution

4. Support for automated processes

an integral part of automated nucleic acid extraction systems

development Trend of 7. Automatic Suction Head

with the development of life science, automated tips are being upgraded in the following directions:

• higher accuracy (micro-upgrade pipetting)
• lower adsorption (improved recovery)
• multi-function integration (temperature control/detection)
• intelligent Adaptation Automation System

automated tips are becoming standard in high-end laboratories.

8. Summary

automated tips determine "experimental accuracy", not simple consumables