15/50mL U-bottom centrifuge tubes

In the laboratory, high-precision experiments such as cell separation, protein extraction and nucleic acid analysis

often relies on the sophisticated design and stable quality of centrifuge tubes

different centrifuge tube designs will have a direct impact on the experimental results

For example, when using a V-bottom centrifuge tube in cell separation experiments, the sediment is easily concentrated at the bottom of the tube.

may result in uneven separation and increased cell loss, which in turn affects the reproducibility of the experiment.

this is also a lot of researchers in the experiment will encounter problems

How to choose the suitable centrifuge tube to better ensure the stability of the experimental data

the 15mL and 50mL U-bottom centrifuge tubes introduced by

Aizin Biology polish the design details based on the requirements of the experimental scenario.

with stable performance, help improve the separation effect and experimental accuracy, adapt to the actual needs of many types of experimental operation.

of

Part 1 Basic information

of Material

High quality medical grade polypropylene (PP), bio-inert, no DNase/RNase contamination

of capacity

15mL and 50mL, covering most conventional centrifugation applications

of

key performance

sterilization by

gamma irradiation, independent sterile packaging, ready to use

of

clear scale

tube height precision printing scale, still clear after high temperature sterilization

of

Part 2 product advantages

with

high resistance to centrifugal force

15mL/50mLU bottom centrifuge tube, the tube body marked with the highest centrifugal force 12000xg,

the

information is clear, the probability of tube explosion is reduced, and the high tolerance of centrifugal force can also meet various experimental needs.

is particularly suitable for large-scale sample processing and high-intensity centrifugation operations to ensure stable and efficient sample processing.

strong sealing

products through-95Kpa negative pressure test, extreme test environment leak-proof, explosion-proof cover, to prevent cross contamination,

protect the centrifuge equipment and ensure the safety of samples.

precise separation, stable and reliable

U-bottom design ensures more uniform sample separation. In cell separation, protein extraction, nucleic acid analysis and other experiments that require precise separation,

the

U-bottom centrifuge tube can ensure that the sample will not be excessively aggregated or deviated during centrifugation, effectively improving the accuracy and reliability of separation.

of

high cleanliness to ensure experimental safety

products undergo strict quality control to ensure sterility, no enzyme, no endotoxin,

comply with international laboratory standards, open the bag ready to use, to ensure the purity of the sample,

is widely used in cytology, molecular biology, clinical testing and other fields.

what are the advantages and of

Part 3 U bottom

precipitation easy resuspension, recovery more stable

the

U-bottom arc structure makes the sedimentation distributed in a thin layer after centrifugation and is not easy to be compacted,

is easier to blow and resuspend after buffer addition, which helps improve overall recovery and reduces mechanical damage to cells and proteins.

Abandoned supernatant is safer and reduces operation error

.

compared with V bottom precipitation is highly concentrated, U bottom precipitation boundary is more clear,

It is not easy to precipitate by aspiration when discarding and cleaning, and can significantly improve the operation fault tolerance rate in multiple washing or batch operations.

to keep the sample state

U-bottom structure to avoid precipitation in the cusp area by strong extrusion, more conducive to maintaining cell morphology and activity,

may also reduce the effect of over-densification of protein precipitation on subsequent solubilization.

more suitable for multi-step experimental process

in the long process experiment of repeated centrifugation, washing and resuspension,

the

U-bottom structure continuously reduces the hidden sample loss in each step, helping to obtain more stable and repeatable experimental results.

The operator is more friendly, the result fluctuates less

.

The U bottom has relatively loose requirements for pipetting angle and tip position, and even if the operator's experience varies greatly,it is not easy to cause significant sample loss due to a single operation error.

Part 4 U bottom vs V bottom centrifuge tube

in the choice of centrifuge tube,U bottom and V bottom design has a clear difference,different design for different experimental needs.

Understanding the advantages and disadvantages of these two designs will help you choose the centrifuge tube that is most suitable for your experiment.

Category U bottom centrifuge tube (new) / V bottom centrifuge tube

precipitation shape forms a thin layer of cake-like precipitation at the base of a broad arc/aggregates into dense dots at the base of a sharp cone

Recycling Convenience The pipette tip can be easily attached to the bottom arc surface,and can be completely recovered by one purge/the sedimentation point is difficult to reach,and repeated flushing and secondary centrifugation are required,which is easy to have residues.

Core advantage High recovery rate (>95%),saving time and reagents,easy operation/fast centrifugal sedimentation

Typical application scenarios Cell collection,protein precipitation,exosome extraction and other precious sample processing requiring high recovery rate/mainly used for rapid sample sedimentation,stratification,and routine separation with low recovery rate requirements

Aizin Guardian Experiment No Worry

Aizin Bio-Centrifuge Tubes are made of USP Class VI polypropylene (PP) material,which is widely used in various experimental applications.

the uniform thickness of the

tube wall ensures the structural stability and reliability of the centrifuge tube,and the high centrifugal force resistance design is more effective to avoid the explosion of the tube.

provide double guarantee for the experiment,we provide a variety of specifications,the capacity range from 0.6~100mL

the

bottom design includes V-bottom,U-bottom and vertical bottom options. In terms of cover type,it covers flat cover,double explosion-proof buckle cover and rotary cover.

and multiple color options for easy management and classification of different samples